Table 3

Three-way permutational multivariate analysis of variance (PERMANOVA) results comparing parameters between cold-acclimated and warm-acclimated normal thyroid and hypothyroid fish
ATa Hb TTc ATa × Hb ATa × TTc Hb × TTc ATa × Hb × TTc

Ucrit

d

F 5.319 12.900 14.768 5.711 0.0088 2.079 0.838
d.f 1, 69 1, 69 1, 69 1, 69 1, 69 1, 69 1, 69
P 0.014* <0.001** <0.001** 0.017* 0.883 0.121 0.351
RMR
F 3.767 1.216 3.069 1.153 0.427 4.479 2.411
d.f 1, 59 1, 59 1, 59 1, 59 1, 59 1, 59 1, 59
P 0.031* 0.273 0.048 0.297 0.653 0.032* 0.103
AMR
F 12.790 8.354 12.430 0.567 1.064 0.112 0.200
d.f 1, 59 1, 59 1, 59 1, 59 1, 59 1, 59 1, 59
P <0.001** 0.004** <0.001** 0.471 0.305 0.829 0.717
MS
F 8.488 9.140 8.251 1.280 0.772 0.352 1.031
d.f 1, 59 1, 59 1, 59 1, 59 1, 59 1, 59 1, 59
P 0.004** 0.004** 0.007** 0.247 0.371 0.637 0.293
LDHe
F 11.547 0.642 145.460 5.611 3.886 0.291 2.346
d.f 1, 72 1, 72 1, 72 1, 72 1, 72 1, 72 1, 72
P <0.001** 0.445 <0.001** 0.018* 0.045* 0.645 0.107
CSe
F 0.505 0.010 45.700 2.410 0.040 0.006 0.246
d.f 1, 72 1, 72 1, 72 1, 72 1, 72 1, 72 1, 72
P 0.497 0.991 <0.001** 0.122 0.935 0.992 0.674
COXe
F 1.920 2.122 16.849 3.872 1.027 0.361 0.406
d.f 1, 56 1, 56 1, 56 1, 56 1, 56 1, 56 1, 56
P 0.159 0.148 <0.001** 0.044* 0.314 0.551 0.548

Abbreviations: AMR, active metabolic rate, CS, citrate synthase; COX, cytochrome oxidase; MS, metabolic scope; LDH, lactate dehydrogenase; RMR, resting metabolic rate.

aAcclimation temperature.

bThyroid treatment.

cTest temperature.

dSwimming performance.

eMaximal activity.

*P<0.05.

**P<0.01.

Little et al.

Little et al. BMC Biology 2013 11:26   doi:10.1186/1741-7007-11-26

Open Data