Additional file 2.

Figure S1. Reducing SDS-PAGE analysis of size exclusion chromatography fractions. Ten micrograms of protein (reduced with DTT) from each size exclusion peak (BioGel P100) were loaded onto a 12% acrylamide NuPage gel. Following electrophoresis, the gel was fixed and stained with 0.1% Coomassie Brilliant Blue R250 using standard methods, destained and photographed. MW standards = Invitrogen Mark 12. Circled faint bands indicate carryover contamination of metalloproteinases (darkest bands) from lanes 2 and 4, respectively. Note that lane 5 is the only peak containing disintegrin bands (dark pair, red bracket); peptides were not visualized and are smaller than the resolution capability of the gel.

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Saviola et al. BMC Biology 2013 11:20   doi:10.1186/1741-7007-11-20