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Open Access Highly Accessed Open Badges Research article

Molecular basis for prey relocation in viperid snakes

Anthony J Saviola1, David Chiszar2, Chardelle Busch2 and Stephen P Mackessy1*

Author Affiliations

1 School of Biological Sciences, University of Northern Colorado, 501 20th St., CB 92, Greeley, CO 80639-0017 USA

2 Department of Psychology, University of Colorado at Boulder, CB 345, Boulder, CO 80309 USA

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BMC Biology 2013, 11:20  doi:10.1186/1741-7007-11-20

Published: 1 March 2013

Additional files

Additional file 1:

Table S1. Raw data: Number of tongue flicks toward envenomated (E) or non-envenomated (NE) mice. This table contains the raw data collected for behavioral experiments 1 and 2. Experiment 1 consisted of paired trials using a non-envenomated vs. and envenomated (whole venom) mouse - this trial was conducted to replicate and confirm past results. Experiment 2 consisted of the same paired trials, but instead of whole venom, one of five size exclusion venom fractions, Peak I, IIa IIb, III or Peptides, was used in "envenomated" mice. Trials were of 10 minutes duration, and the number of tongue flicks directed toward one or the other mouse was recorded.

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Additional file 2:

Figure S1. Reducing SDS-PAGE analysis of size exclusion chromatography fractions. Ten micrograms of protein (reduced with DTT) from each size exclusion peak (BioGel P100) were loaded onto a 12% acrylamide NuPage gel. Following electrophoresis, the gel was fixed and stained with 0.1% Coomassie Brilliant Blue R250 using standard methods, destained and photographed. MW standards = Invitrogen Mark 12. Circled faint bands indicate carryover contamination of metalloproteinases (darkest bands) from lanes 2 and 4, respectively. Note that lane 5 is the only peak containing disintegrin bands (dark pair, red bracket); peptides were not visualized and are smaller than the resolution capability of the gel.

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