Additional file 2.

Figure S1. Determination of the mCherry reporter mRNA and protein half-lives. (A) Quantitative reverse transcription PCR measurement of mCherry mRNA decay after actinomycin D treatment in two different clones of the 6C2 cell line. The best-fitting exponential curve (black line) was found by minimizing least squares (between exponential curve and biological data). The deduced mCherry mRNA half-life was 7 hours and 4 minutes (424 minutes). (B) Flow-cytometry measurement of mCherry protein fluorescence decay after cycloheximide treatment in two different clones of the 6C2 cell line. The best-fitting exponential curve (black line) was found by minimizing least squares (between exponential curve and biological data). The deduced mCherry protein half-life was 65 hours and 47 minutes (3,947 minutes). For both parts, ordinates are on a logarithmic scale.

Format: PDF Size: 115KB Download file

This file can be viewed with: Adobe Acrobat Reader

ViƱuelas et al. BMC Biology 2013 11:15   doi:10.1186/1741-7007-11-15