Exploration of model parameters based on treatments with chromatin-modifying agents. (A) Evolution of mean fluorescence intensity following kinetics of treatment with trichostatin A (TSA; solid line) and 5-azacytidine (5-AzaC; dotted line) (0 to 48 hours) for three cellular clones. (B) Distributions of the plausible chromatin dynamics. For each clone, all 114 possible couples of (1/kon; 1/koff) values were plotted, expressed as mean open time (1/koff) and mean closed time (1/kon), after removal of all parameter sets that were not able to account for the transcription-translation dynamics under TSA and 5-AzaC treatments. (C) This experiment was the same as for (B), except than the transcription rate (ρ) and the mean open time (1/koff) parameters were reduced to a single effective parameter (ρ/koff), representing the mean burst size. min, minutes; h, hours; d, days; m, months.
Viñuelas et al. BMC Biology 2013 11:15 doi:10.1186/1741-7007-11-15