Assessment and quantification of follicle types in the ovaries of prepubertal Amhr2-Cre/+;Notch2flox/- and control littermate mice. (A-D) Follicle types were assessed and counted in serial sections of ovaries isolated from Amhr2-Cre/+;Notch2flox/- (white bars) and control littermate (black bars) mice at postnatal day 18. (A-C) Amhr2-Cre/+;Notch2flox/- ovaries exhibited a significant increase in the number of multi-oocyte follicles (containing four or more oocytes) of (A) the primordial, (B) primary and (C) secondary follicle types compared with littermate control ovaries. Amhr2-Cre/+;Notch2flox/- ovaries also exhibited a significantly reduced number of primordial follicles containing a single oocyte. * P < 0.05; ** P < 0.005.
Xu and Gridley BMC Biology 2013 11:13 doi:10.1186/1741-7007-11-13