Translation states (TL) in the pab2 pab8 poly(A) binding protein mutant. Δlog2 TL indicates translational stimulation (>0) or inhibition (<0) in the pab2 pab8 mutant as compared to wild-type. (A) Volcano plot showing the distribution of alterations in ribosome occupancy (x-axis) versus the false discovery rate (FDR, y-axis). Many of the translationally stimulated mRNAs encode seed storage proteins. The color coding distinguishes mRNAs with FDR-validated significance (red for upregulated, black for downregulated) from mRNAs showing trends only (green for upregulated, orange for downregulated). Genes above the stippled line are less than 5% likely to be false positives. (B-E) The effect of the pab mutation on ribosome occupancy is related to mRNA length (B), specifically the lengths of the coding sequence (C) and 5′ untranslated region (UTR) (D), rather than the 3′ UTR (E). Asterisks indicate a significant difference compared to the gray 'no change’ bin (Student t test); **P <0.0001; * ≥ 0.0001 P <0.05. Error bars are standard errors of the mean.
Tiruneh et al. BMC Biology 2013 11:123 doi:10.1186/1741-7007-11-123