Complementation of the ΔipsA phenotype with ipsA and the target gene ino1 (cg3323). For chromosomal complementation, the gene ipsA was integrated into the intergenic region of cg1121 to cg1122 under control of the native promoter. (A) Growth of the strains in CGXII minimal medium with 2% (w/v) glucose as carbon source. The average and standard deviation of three biological replicates is shown. (B) Fluorescence microscopy images of stationary phase cells of the cultures presented in (A). (C) Plasmid-based complementation with the target gene ino1 (cg3323). The strains were cultivated in CGXII minimal medium with 2% (w/v) glucose and 50 μM isopropyl β-D-1-thiogalactopyranoside (IPTG) for induction of ino1 expression.
Baumgart et al. BMC Biology 2013 11:122 doi:10.1186/1741-7007-11-122