Quantitative reverse transcription PCR (qRT-PCR) analysis and comparison of freezing tolerance between WT and mutant Arabidopsis. (a) matured miR398 expression under acclimation at 16°C and 4°C; (b)CSD1 and CSD2 expression at 16°C; (c) qRT-PCR analysis of primary transcript MIR398 a, b and c during acclimation at 16°C; (d) RT-PCR analysis of expression of MIR398c, CSD1 and CSD2 between WT Arabidopsis and the mutants; (e)Arabidopsis treated at −9°C after 21 days of recovery growth following 16°C/4°C/non-acclimation conditions; (f) Electrolyte leakage (EL) of Arabidopsis leaves at 1 hour after treatment at −9°C. WT, wild type.
Chen et al. BMC Biology 2013 11:121 doi:10.1186/1741-7007-11-121