Eukaryotic expression and EMSA assays of CdICE1 and transcriptional activation assays. (a) Eukaryotic expression of CdICE1 and purification using His-tag; (b) Binding assays between CdICE1 protein and MYC elements: purified protein was incubated with 50 ng of biotin-labeled probe. Non-labeled probe with different concentrations (50, 100, and 500 times) was added; (c) Fragment constructs derived from different N’-deletions of amino acids; (d) Transcriptional activation assays of CdICE1 via yeast one-hybrid assays. EMSA, electrophoretic mobility shift assay.
Chen et al. BMC Biology 2013 11:121 doi:10.1186/1741-7007-11-121