Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398
- Equal contributors
1 College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
2 Grass Research Centre, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, Jiangsu China
BMC Biology 2013, 11:121 doi:10.1186/1741-7007-11-121Published: 19 December 2013
Additional file 1: Figure S1:
Analysis of the promoter sequence of CdICE1 and responsive elements. Note: Functional elements as predicted by PLACE software (http://www.dna.affrc.go.jp/PLACE/signalscan.html webcite) are either underlined or shaded.
Format: TIFF Size: 953KB Download file
Additional file 2: Figure S2:
PCR identification of resistant T1 generation plants. (a) PCR assays for CdICE1 at the genome level; (b) QRT-PCR assays of CdICE1 expression in transgenic plants.
Format: TIFF Size: 264KB Download file
Additional file 3: Figure S3:
Prediction of the promoter sequence of MIR398b and MIR398c and responsive elements. Note: Functional elements as predicted by PLACE software (http://www.dna.affrc.go.jp/PLACE/signalscan.html webcite) are underlined.
Format: TIFF Size: 220KB Download file
Additional file 4: Figure S4:
ROS assays in WT and CdICE1 overexpressing plants under 16°C. (a) O2– content; (b) H2O2 content. Asterisk indicates significant difference at P <0.05 compared with the WT plants by Duncan’s test.
Format: TIFF Size: 81KB Download file