Open Access Research article

Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398

Yu Chen12, Jiafu Jiang1, Aiping Song1, Sumei Chen1, Hong Shan1, Huolin Luo1, Chunsun Gu1, Jing Sun1, Lu Zhu1, Weimin Fang1 and Fadi Chen1*

Author Affiliations

1 College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China

2 Grass Research Centre, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, Jiangsu China

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BMC Biology 2013, 11:121  doi:10.1186/1741-7007-11-121

Published: 19 December 2013

Additional files

Additional file 1: Figure S1:

Analysis of the promoter sequence of CdICE1 and responsive elements. Note: Functional elements as predicted by PLACE software (http://www.dna.affrc.go.jp/PLACE/signalscan.html webcite) are either underlined or shaded.

Format: TIFF Size: 953KB Download file

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Additional file 2: Figure S2:

PCR identification of resistant T1 generation plants. (a) PCR assays for CdICE1 at the genome level; (b) QRT-PCR assays of CdICE1 expression in transgenic plants.

Format: TIFF Size: 264KB Download file

Open Data

Additional file 3: Figure S3:

Prediction of the promoter sequence of MIR398b and MIR398c and responsive elements. Note: Functional elements as predicted by PLACE software (http://www.dna.affrc.go.jp/PLACE/signalscan.html webcite) are underlined.

Format: TIFF Size: 220KB Download file

Open Data

Additional file 4: Figure S4:

ROS assays in WT and CdICE1 overexpressing plants under 16°C. (a) O2 content; (b) H2O2 content. Asterisk indicates significant difference at P <0.05 compared with the WT plants by Duncan’s test.

Format: TIFF Size: 81KB Download file

Open Data