Lung phenotype after mesenchymal Sox9 knockout. (A) Pups were collected at birth. Mutant pups died less than an hour after birth, and showed gasping, retractions, and cyanosis. (B) Mutant pups did not survive post-natally. (C) Despite the respiratory mortality phenotype, no change in weight at birth was observed between the wild-type pups and the mutant pups. (D-I) Samples of lung at embryonic day (E) 18.5 were collected and analyzed. (E)Sox9Δ/Δ did not show obvious altered lung branching compared with (D) the control. (F, G) Alcian blue staining of (F) E18.5 wild-type and (G) mutant lungs: normal tracheal rings were observed in the control normal lung, Sox9 knockout trachea revealed no tracheal rings. (H, I) Transverse section of E18.5 trachea was stained with Alcian blue. (H) Wild-type and (I) mutant mice.
Turcatel et al. BMC Biology 2013 11:117 doi:10.1186/1741-7007-11-117