The number of DNA double strand breaks determines the probability of a p53 pulse. (A) Schematic representation of the experimental approach. Cells treated with low doses of NCS (0 to 100 ng/ml) were imaged for 8hr. Induced DSBs and p53 dynamics were quantified. (B) On the resulting data, we performed a robust linear regression (blue line) and calculated the corresponding confidence interval (α = 0.05, shaded blue area). For visualization, cells were binned according to the number of induced DSBs (bin size W = 4 foci) and the fraction of cells inducing a p53 pulse was plotted for each bin (blue dots). The number of cells in each bin is indicated by grey bars (total number of cells = approximately 350). DSBs, couble strand breaks; NCS, neocarcinostatin.
Loewer et al. BMC Biology 2013 11:114 doi:10.1186/1741-7007-11-114