Additional file 5: Figure S4.

Distribution of Delta nascent transcripts in an early stage 5 embryo. A1 Flat mount of an embryo at the 41 LBS stage, hybridized with a Delta probe that detects unprocessed nascent transcripts much more strongly than spliced cytoplasmic transcripts (see Additional file 2: Figure S1). At this stage Delta transcripts are no longer detectable in the peri-proctodeal region. Two posterior stripes of Delta expression remain, defined by an accumulation of cytoplasmic transcript, but nuclear dots of nascent transcript are no longer detectable in these stripes (insert A4). Nuclear dots show that transcription of Delta is activated at high level in more anterior medial cells (magenta arrowheads in insert A3). These cells are neuronal precursors. In more mature segments they will form clusters where Delta is present at high level in both the nuclei and the cytoplasm (insert A2). The head is to the left. Black arrowhead: mandibular segment; an asterisk marks the proctodeum. Scale bar in A1: 200 μm, in A2-4: 20 μm.

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Brena and Akam BMC Biology 2013 11:112   doi:10.1186/1741-7007-11-112