Additional file 4: Figure S3.

Distribution of Delta nascent transcripts in an early stage 4 embryo. A1 Flat mount of an embryo at the 15 LBS stage, hybridized with a Delta probe that detects unprocessed nascent transcripts much more strongly than spliced cytoplasmic transcripts (see Additional file 2: Figure S1). At this stage nuclear dots of nascent transcript (magenta arrowheads) are still prominent in many cells of the peri-proctodeal region (enlarged in A4, A5), although the signal intensity may be somewhat lower than in earlier stages. In more mature Delta stripes, transcript is still present at high level in the cytoplasm, but nuclear dots are less frequent (insert A3). Nuclear dots are almost undetectable in the oldest, more anterior stripes (insert A2). The head is to the left. Black arrowhead: mandibular segment; an asterisk marks the proctodeum. Scale bar in A1: 200 μm, in A2-5: 20 μm.

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Brena and Akam BMC Biology 2013 11:112   doi:10.1186/1741-7007-11-112