Open Access Methodology article

Spatial-temporal targeting of lung-specific mesenchyme by a Tbx4 enhancer

Wenming Zhang1, Douglas B Menke2, Meisheng Jiang3, Hui Chen1, David Warburton1, Gianluca Turcatel1, Chi-Han Lu1, Wei Xu1, Yongfeng Luo1 and Wei Shi1*

Author Affiliations

1 Developmental Biology and Regenerative Medicine Program, Department of Surgery, Children’s Hospital Los Angeles, Keck School of Medicine, University of Southern California, 4650 Sunset Blvd., MS 35, Los Angeles, CA 90027, USA

2 Department of Genetics, University of Georgia, Athens, GA 30602, USA

3 Department of Molecular and Medical Pharmacology, Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA

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BMC Biology 2013, 11:111  doi:10.1186/1741-7007-11-111

Published: 13 November 2013

Additional files

Additional file 1:

Lung neuroendocrine cells were not targeted by the Tbx4 lung enhancer, shown by co-immunostaining of GFP (green) and CGRP (red) for E18.5 lung tissue section of the triple transgenic mouse (Tbx4-rtTA/TetO-Cre/mT-mG) with Dox induction from E6.5 to E18.5. Blue: DAPI nuclear counterstaining.

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Additional file 2:

Cells with Tbx4 lung enhancer activity in adult lungs. (A) Adult mice with triple transgenic genotypes (Tbx4-rtTA/TetO-Cre/mT-mG) were induced by Dox administration for two weeks. The lung tissue sections were co-immunostained with GFP and one of the cell markers as indicated. Cells with GFP expression (green) were not positive for T1α and PECAM-1 (red). However, most GFP-positive cells were positive for NG2, and a few GFP-positive cells were positive for SMA, shown by overlapped co-staining (yellow color). DAPI was used for nuclear counterstaining (blue). (B) Evaluation of adult transgenic reporter mouse lungs in the absence of Dox induction. Mouse genotypes are indicated above the panel. Expression of endogenous mTomato (red) and mGFP (green) was examined for the lung frozen sections under fluorescence microscope. DAPI was used for nuclear counterstaining (blue).

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