Additional file 1: Figure S1.

Wnt6KO flies have no detectable Wnt6 mRNA. (A) Quantitative PCR on genomic DNA from control and Wnt6KO flies confirming absence of the Wnt6 knockout region, but not absence of two other unrelated genomic regions (mir-278 and sty), in the knockout flies. (B) Quantitative RT-PCR to detect Wnt6 mRNA levels in control and Wnt6 knockout flies reveals no Wnt6 transcript left in the knockouts. (C-D’) In situ hybridization to detect Wnt6 mRNA in wing discs (C-C’) and eye-antenna discs (D-D’) of control and Wnt6 knockout animals.

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Doumpas et al. BMC Biology 2013 11:104   doi:10.1186/1741-7007-11-104