Additional file 1: Figure S1.

Drosophila dZip1 and dZip2 are two adjacent Zip family members involved in zinc uptake. (A) Phylogenetic tree revealing the relationship between human and Drosophila Zip members. All human Zips were used individually as queries in a series of BLASTP searches in the genome of D. melanogaster. The tree was generated by using ClustalX (version 1.81) and displayed in Treeview. This tree analysis result is consistent with that of a previous report.17(B) Relative genomic location of dZip1 (CG9428) and dZip2 (CG9430). Both genes are located at postion 42C6 of the chromosome 2 in Drosophila melanogaster, suggesting a recent evolutionary duplication. (C) Under conditions of zinc deficiency (0.3 mmol/I EDTA), there was impaired development of larvae after ubiquitous knockdown by RNA interference (RNAi): dZip2 RNAi and dZip1 dZip2 double RNAi (dZip1, dZip2 RNAi). The genotypes of flies are da-GAL4/+ for the control and da-GAL4/dZip1-RNAi for dZip1-RNAi, da-GAL4/dZip2-RNAi for dZip2-RNAi, and da-GAL4/dZip1-, dZip2-RNAi for the double RNAi fly. Data are presented as means ± SEM; n≥3. *P<0.05, **P<0.01, ***P<0.001; one-way ANOVA.

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Qin et al. BMC Biology 2013 11:101   doi:10.1186/1741-7007-11-101