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Open Access Research article

Functional studies of Drosophila zinc transporters reveal the mechanism for dietary zinc absorption and regulation

Qiuhong Qin, Xiaoxi Wang and Bing Zhou*

Author Affiliations

State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, China

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BMC Biology 2013, 11:101  doi:10.1186/1741-7007-11-101

Published: 24 September 2013

Additional files

Additional file 1: Figure S1:

Drosophila dZip1 and dZip2 are two adjacent Zip family members involved in zinc uptake. (A) Phylogenetic tree revealing the relationship between human and Drosophila Zip members. All human Zips were used individually as queries in a series of BLASTP searches in the genome of D. melanogaster. The tree was generated by using ClustalX (version 1.81) and displayed in Treeview. This tree analysis result is consistent with that of a previous report.17(B) Relative genomic location of dZip1 (CG9428) and dZip2 (CG9430). Both genes are located at postion 42C6 of the chromosome 2 in Drosophila melanogaster, suggesting a recent evolutionary duplication. (C) Under conditions of zinc deficiency (0.3 mmol/I EDTA), there was impaired development of larvae after ubiquitous knockdown by RNA interference (RNAi): dZip2 RNAi and dZip1 dZip2 double RNAi (dZip1, dZip2 RNAi). The genotypes of flies are da-GAL4/+ for the control and da-GAL4/dZip1-RNAi for dZip1-RNAi, da-GAL4/dZip2-RNAi for dZip2-RNAi, and da-GAL4/dZip1-, dZip2-RNAi for the double RNAi fly. Data are presented as means ± SEM; n≥3. *P<0.05, **P<0.01, ***P<0.001; one-way ANOVA.

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Additional file 2: Figure S2:

Phylogenetic analysis of ZnTs in Drosophila (compared with those in human) and the RNA interference (RNAi) effects of some dZnT lines. RNAi lines were obtained from the Vienna Drosophila RNAi Center (VDRC) or custom-made in the Tsinghua Fly Center. (A) Phylogenetic tree revealing the relationship between human and Drosophila ZnT members. The predicted intracellular ZnTs are in pink. All human ZnTs were used individually as queries in a series of BLASTP searches of the genome of Drosophila melanogaster. The tree was generated by using ClustalX (version 1.81) and displayed n Treeview. (B-D) Reverse transcriptase (RT)-PCR analysis of the gut-specific knockdown effect of RNAi lines of (B)CG11163 and (C)CG8632. (D) Expression of CG31860 was not detected in the gut. rp49 was used as the loading control. Analysis of CG6672 (dZnT7) and CG5130 (dZnT1h) are described in Figures 4 and 5 respectively.

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Additional file 3: Table S1:

Analysis of intracellular ZnTs for their roles in dietary zinc absorption in Drosophila. (A) Phenotypes of individual RNA interference (RNAi) lines driven by da-GAL4, a ubiquitous GAL4 line, under normal or zinc-limited conditions. (B) Phenotypic analysis of midgut-specific knockdown of individual RNAi lines on normal or zinc-limited food. NP3084 was used as the midgut GAL4 driver.

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