Additional file 2: Figure S2.
Strains could be ranked according to polyQ aggregation. (a) Lack of correlation between polyQ-YFP expression levels in L1 animals and its aggregation across the recombinant inbred lines (RILs). Aggregation data are the same as in Figure 2b. Data points in color correspond to the selected RILs and parental strains as in panel (b). (b) Statistical analysis of polyQ aggregation in selected strains. Each symbol represents an individual animal, and data are the same as in Figure 2b. RIL 16 represents a suppressed aggregation phenotype, ranked as 1 for aggregation; RIL 10 is similar to Q40Bristol, both ranked as 2 to 3; RIL 2 is similar to Q40DR1350, ranked as 4 to 5; and RILs 12(2) and 15 represent an enhanced aggregation phenotype, ranked as 6 to 7. Data were analyzed by ANOVA followed by Bonferroni’s multiple comparison test (all pair combinations), α = 0.01. The indicated pairs of strains are not significantly different from each other, and all other combinations are P<0.001. (c) Ratio of polyQ-yellow fluroescent protein (YFP) in SDS-resistant high molecular weight and soluble monomeric species.
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Gidalevitz et al. BMC Biology 2013 11:100 doi:10.1186/1741-7007-11-100