Additional file 5.

Cellular dynamics during rosette formation and its developmental plasticity. (A) Cortex rupture and bleb formation in Ea/Ep. (Top) Side view of two-dimensional (2D) confocal slices. White arrows point to breakpoints in the cortex, while the red arrow marks a reemerging NMY-2 focus at the tip of the bleb. (Bottom left) Schematic representation of blebbing events in a representative embryo. Blebs are indicated by arrows, and the dashed white line indicates the initial position of Ea/Ep apices. (Bottom right) Size distribution of blebs shown on the left. (B) Effect of non-muscle myosin (NMY)-2 RNA interference (RNAi) on blebbing and internalization dynamics. (Top) Cortical views overlaying cellular membranes (gray) with color-coded NMY-2 intensities. (Right) Covering kinetics and number of blebs in wild-type and nmy-2 RNAi-treated embryos. (C) Cells in rosettes divided along the long axis. (First panel) three-dimensional (3D)projection image shows polarization of cells in rosettes. (Second panel) Quantification of long and short axis of cells from 3D still images (mean ± SD, n = 5). (Third panel) Cell polarization during rosette formation. (Fourth panel) Division orientation in the same embryos as in third panel (double arrows) (n = 5). (D) Cellular topology during endoderm internalization in three different nematodes. Color code for tissues is indicated on the bottom left, and direction of covering with black arrows.

Format: PNG Size: 1.3MB Download file

Pohl et al. BMC Biology 2012 10:94   doi:10.1186/1741-7007-10-94