Additional file 4.

Covering depended on surrounding cells. Irradiation of(A) P3 or (B) other neighboring AB cells prevented Ea/Ep internalization. (Top panels) Time-lapse microscopy with three-dimensional (3D) projection still images. Lightning marks the irradiated cells. Mesoderm is outlined with dashed lines, non-internalized endoderm is marked with a white circle, and arrow points to the center of the rosette. (Bottom panels) Schematic illustration depicting the embryo in the same orientation as above, and highlighting the irradiated cell.

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Pohl et al. BMC Biology 2012 10:94   doi:10.1186/1741-7007-10-94