Figure 5.

Principal component analysis (PCA) of the 49 strains by 8 feature matrices to identify strains behaving differently from N2. (A) Positions of 49 mutants in the 6-dimensional feature (principal component) space between mutants projected onto 2 dimensions by classical multidimensional scaling for the indicated ΔT. N2 is shown in orange. In red are strains that are 4 distance units away from N2 and are predicted to be different from N2 by bootstrapping. In blue are strains predicted to be different from N2 by bootstrapping and are less than 4 units away from N2 in the two-dimensional space. In black are strains that are not predicted to be different from N2 by bootstrapping and are less than 4 units away from N2 in the two-dimensional space. (B) Position of different strains from N2 ordered by increasing distance from N2 (dN2) calculated from the graphs in (A). The colors are as described in (A). Vertical dashed line represents 4 distance units away from N2.