Table 2

Confidence score of homologs from Swiss-Prot database.

Evaluation method

Criteria

Points


BLAST e-value

< 0.001

1


Sequence identity between the query and the hit

identity 30% to 50%, coverage > 40%

1

identity 50% to 70%, coverage > 40%

2


identity 70% to 90%, coverage > 40%

3


identity 90% to 99%, coverage > 40%

4


identity > 99%, coverage > 40%

5


BLAST alignment coverage for both query and hit

60% to 80%

1


80% to 100%

2


The query against the proteome associated with the hit

Best hit

2


N/A

1


The hit against the proteome associated with the query

Best hit

2


N/A

1


Cong and Grishin BMC Biology 2012 10:82   doi:10.1186/1741-7007-10-82

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