Table 2

Confidence score of homologs from Swiss-Prot database.

Evaluation method

Criteria

Points

BLAST e-value

< 0.001

1

Sequence identity between the query and the hit

identity 30% to 50%, coverage > 40%

1

identity 50% to 70%, coverage > 40%

2

identity 70% to 90%, coverage > 40%

3

identity 90% to 99%, coverage > 40%

4

identity > 99%, coverage > 40%

5

BLAST alignment coverage for both query and hit

60% to 80%

1

80% to 100%

2

The query against the proteome associated with the hit

Best hit

2

N/A

1

The hit against the proteome associated with the query

Best hit

2

N/A

1

Cong and Grishin BMC Biology 2012 10:82   doi:10.1186/1741-7007-10-82