Tamoxifen-inducible recombination in double transgenic CaMKIIα-CreERT2/CAG-loxP.EGFP rats. Brain slices were immunostained for (A,D,G) Cre recombinase or (B,C,E,F,H,I) EGFP expression. Tamoxifen (+Tx)-induced EGFP (B,E,H) expression mirrors Cre expression (A,D,G), whereas uninduced animals (-Tx) only show low background recombination (C,F,I). (J-O) Brain slices of tamoxifen-induced CaMKIIα-CreERT2/CAG-loxP.EGFP rats were double-stained for NeuN and EGFP. Co-localization of EGFP and NeuN demonstrated successful Cre-mediated recombination in neurons of the cortex (L) and the CA1 region of the hippocampus (O). Scale bars: J-l 100 μm, M-O 50 μm. EGFP: enhanced green fluorescent protein.
Schönig et al. BMC Biology 2012 10:77 doi:10.1186/1741-7007-10-77