CaMKIIα-CreERT2-mediated recombination in the rat brain. Functional recombination using different CaMKIIα-CreERT2 founder lines was assessed with tamoxifen-induced double transgenic CaMKIIα-CreERT2/CAG-loxP.EGFP rats. Double transgenic rats were injected with tamoxifen for five consecutive days. Ten days after the last injection, brain sections were prepared and analyzed by EGFP immunohistochemistry. EGFP expression, which indicates Cre-mediated recombination, is restricted to forebrain structures. Recombination efficiency is dependent on the Cre driver lines used. (A) CaMKIIα-CreERT2 line 327, (B) line 396, (C) line 408 and (D) line 404. EGFP: enhanced green fluorescent protein.
Schönig et al. BMC Biology 2012 10:77 doi:10.1186/1741-7007-10-77