Conditional gene expression systems in the transgenic rat brain
- Equal contributors
1 Department of Molecular Biology, Central Institute of Mental Health and Heidelberg University, Medical Faculty Mannheim, J5, 68159 Mannheim, Germany
2 Department of Addictive Behavior and Addiction Medicine, Central Institute of Mental Health, Heidelberg University, J5, 68159 Mannheim, Germany
3 German Cancer Research Center (DKFZ), Molecular Immunology, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
4 Zentrum für Molekulare Biologie Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
BMC Biology 2012, 10:77 doi:10.1186/1741-7007-10-77Published: 3 September 2012
Additional file 1:
CreERT2 protein expression in the brain of transgenic CaMKIIα-CreERT2 rats. CaMKIIα-CreERT2 rats were injected twice within 12 hours with tamoxifen for nuclear localization of the Cre recombinase. Three hours after the second injection, animals were prepared for analysis. Brain sections were immunostained using a Cre antibody. The most prominent Cre immunoreactivity was found in hippocampal pyramidal neurons (B,C,E,G) and in cortical structures. CaMKIIα-CreERT2 line 327 (A,B); line 396 (C,D); line 408 (E,F) and line 404 (G,H).
Format: TIFF Size: 14.5MB Download file
Additional file 2:
Constitutive β-galactosidase expression in transgenic pCAG-loxP.EGFP Cre reporter rats. Macroscopic appearance of X-Gal-stained tissues revealed strong b-galactosidase expression in the lung, kidney, muscle, heart, spleen and gastrointestinal tract including appendix and pancreas.
Format: TIFF Size: 2.6MB Download file