Additional file 1.

Figure S1. Cytokine pathway was enriched in MCF10ATw relative to MCF10A cells. (A) Enrichment plot of changes in mRNA levels of cytokines included in the Biocarta_Cytokine_Pathway in MCF10ATw versus MCF10A cells. Profile of the running ES Score and positions of gene set members on the ranked list of genes are shown. (B) Heat map of cytokine expression in MCF10ATw versus MCF10A cells. Microarray data were performed in triplicate. Each row represents relative expression levels of a gene and each column represents one replicate of MCF10A or MCF10ATw cells. Red and blue indicate up-regulated or down-regulated expression, respectively. (C) Relative mRNA levels normalized to β-ACTIN (left) (See Methods) and secreted IL8 per 8 × 105 cells (right) from MCF10A and MCF10ATw cells. (D) Details of the GSEA analysis for the Biocarta_Cytokine_Pathway.

Format: PDF Size: 379KB Download file

This file can be viewed with: Adobe Acrobat Reader

Li et al. BMC Biology 2012 10:73   doi:10.1186/1741-7007-10-73