Table 1

buffy mutant larvae maintain smaller steady-state lipid and glycogen stores

Wild-type

buffyH37

buffyH37/wt


Complete medium:

Nile Red fat body (mean luminosity)

62.4 ± 3.6 (3)

50.4 ± 2.5 (3)

0.81

Nile Red lysate (FU)

323 ± 47 (4)

260 ± 16 (4)

0.80

Triacylglyceride (μg TAG/μg protein)

0.56 ± 0.05 (4)

0.47 ± 0.04 (4)

0.85

Glycogen (ng glycogen/μg protein)

1.54 ± 0.16 (6)

1.30 ± 0.11 (5)

0.85

20% medium:

Nile Red fat body (mean luminosity)

62.2 ± 15.5 (3)

46.3 ± 1.6 (3)

0.74

Number of lipid droplets/fat body cell

30.7 ± 5.3 (3)

20.7 ± 3.4 (3)

0.67

Perimeter of lipid droplets

38.9 ± 3.4 (3)

32.3 ± 2.2 (3)

0.83

Complete medium + added sucrose:

Triacylglyceride (μg TAG/μg protein)

0.87 ± 0.07 (4)

0.74 ± 0.10 (4)

0.85


All experiments were performed on third instar larvae fed the indicated medium. All data are presented as averages ± SEM with number of biological replicates in parentheses. With the exception of mean luminosity of Nile Red fat body in complete medium (P = 0.05), all other differences are not significant (P > 0.1) as determined by Student's unpaired two-tailed t test. Nevertheless, the trend is similar across all measurements. For TAG assays, a representative of four biological replicates is presented. For technical reasons the mean luminosity cannot be compared across different media conditions (see Methods for experimental details).

Monserrate et al. BMC Biology 2012 10:63   doi:10.1186/1741-7007-10-63

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