Additional file 1.

Figure S1. (A) Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) demonstrating knockdown of buffy transcript levels in two lines in which buffy RNAi was expressed using the ubiquitous driver daughterless-Gal4. (B-D) Fat body Nile Red stain of RNAi lines reared in restrictive media (20% cornmeal/yeast/agar food (CY); 1.8% sucrose, compare to Figure 1D) phenocopies observations made in the buffyH37 null mutant. (B) da-Gal4 driver alone; (C) da-Gal4, UAS-buffyRNAi498; (D) da-Gal4, UAS-buffyRNAi499. (E-G) LysoTracker Red (LTR) stain on fat bodies from RNAi lines amino-acid starved for 2 h also phenocopies the buffyH37 null mutant. (E) da-Gal4 driver alone; (F) da-Gal4, UAS-buffyRNAi498; (G) da-Gal4, UAS-buffyRNAi499 (compare to Figure 4A).

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Monserrate et al. BMC Biology 2012 10:63   doi:10.1186/1741-7007-10-63