Open Access Research article

Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assembly pathway

Eeshita G Dastidar12, Guillem Dayer3, Zoe M Holland4, Dominique Dorin-Semblat34, Aurélie Claes12, Arnaud Chêne12, Amit Sharma5, Romain Hamelin6, Marc Moniatte6, Jose-Juan Lopez-Rubio12, Artur Scherf12* and Christian Doerig347*

Author Affiliations

1 Biology of Host-Parasite Interactions Unit, Institut Pasteur, 25 rue du Dr. Roux, F-75724 Paris, France

2 CNRS URA2581, 25 rue du Dr. Roux, F-75724 Paris, France

3 Inserm-EPFL Joint Laboratory, Global Health Institute, EPFL-SV-GHI, Station 19, CH-1015 Lausanne, Switzerland

4 Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, Glasgow G12 8TA, Scotland, UK

5 International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110 067 New Delhi, India

6 Proteomics Core Facility, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland

7 Department of Microbiology, Building 76, Monash University, Wellington Road, Clayton, VIC 3800, Australia

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BMC Biology 2012, 10:5  doi:10.1186/1741-7007-10-5

Published: 31 January 2012

Additional files

Additional file 1:

Additional Table A1. Table of primers used in this study.

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Additional file 2:

Additional Figures A1-A6. Additional Figures A1-A6 and their legends.

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Additional file 3:

Additional Table A2. List of proteins identified by mass spectrometry analysis of protein complexes immunoprecipitated from the parasite line expressing hemagglutinin-tagged PfCK2β1.

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Additional file 4:

Additional Table A3. List of proteins identified by mass spectrometry analysis of protein complexes immunoprecipitated from the parasite line expressing hemagglutinin-tagged PfCK2β2.

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Additional file 5:

Additional Table A4. Mass spectrometry data from immunoprecipitates obtained with PfCK2β1 and PfCK2β2 hemagglutinin-tagged transgenic lines, highlighting the significant overlap in the identity of recovered proteins, and the occurrence of potential interactors that are unique to either PfCK2 regulatory subunit. Note that the data are not sufficient to draw statistically supported conclusions about the possible restriction of proteins to the interactome list of one or the other β subunit, and that this table must be considered as a preliminary dataset only.

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