Additional file 4.
Movie 4 - Small endocytic vesicle hot-spots triggered by filopodial - lamellipodial contacts. This representative time-lapse movie demonstrates membrane retrieval triggered by contact between a filopodium and lamellipodium. A focal pulse of FM 5-95 transiently labeled the growth cone surface membrane and confocal images were acquired as described in Movie 1. The yellow arrow (00:09) indicates filopodial detachment, subsequent contact with the growth cone dorsal surface, and the formation of numerous small endocytic vesicles (00:21 to 00:27). Movie 4 corresponds to Figure 4A. Scale bar, 5 μm. Format: MOV (MPEG4 compression).
Format: MOV Size: 2.9MB Download file
Hines et al. BMC Biology 2012 10:4 doi:10.1186/1741-7007-10-4