Additional file 10.
Movie 10 - Endocytic vesicle formation at self-membrane contacts requires F-actin. This time-lapse movie shows a representative example of the focal membrane labeling assay performed on a growth cone pre-treated with cytochalasin D (30 nM). Movie 10 corresponds to Figure 7C and 7 the time (minutes:seconds) is indicated at the top left. A filopodium contacts a nearby region of the growth cone periphery (yellow arrow, 00:21 to 00:26). This self-contact fails to elicit vesicle formation at this location. Surface labeling and image acquisition was performed as described in Movie 1. Scale bar, 5 μm. Format: MOV (MPEG4 compression).
Format: MOV Size: 3.6MB Download file
Hines et al. BMC Biology 2012 10:4 doi:10.1186/1741-7007-10-4