Additional file 1.
Movie 1 - Small endocytic vesicle hot-spots at dorsal ridges of the growth cone. These representative time-lapse movies show examples of rapid membrane retrieval triggered at dorsal ridges. A focal pulse of FM 5-95 transiently labeled the plasma membrane (evident at 00:00). The brief dye pulse was delivered from a micropipette positioned 100 μm in front of the leading edge of the growth cone, which is indicated by the top arrow in frame 1. Confocal images were collected (1 Hz) during the endocytic assay and the time frames (minutes:seconds) are indicated at the top left. Movie 1 corresponds to Figure 2A, B. The scale bar (5 μm) applies to each example. Format: MOV (MPEG4 compression).
Format: MOV Size: 4.5MB Download file
Hines et al. BMC Biology 2012 10:4 doi:10.1186/1741-7007-10-4