Additional file 3.

BMP2 and mechanical load synergistically regulate Id1 gene expression in primary human mesenchymal stem cells. Data of one representative experiment is depicted. Human primary mesenchymal stem cells (hMSCs) were embedded in fibrin gels and loaded for 3 days in the absence or presence of 10 nM BMP2. Embedding and loading was performed as described previously [68]. Total RNA was extracted and Id1 gene expression was analysed by qRT-PCR.

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Kopf et al. BMC Biology 2012 10:37   doi:10.1186/1741-7007-10-37