Research article
Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress
1 Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-41296 Göteborg, Sweden
2 Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd. Tech E136, Evanston, IL 60208, USA
BMC Biology 2012, 10:16 doi:10.1186/1741-7007-10-16
Published: 1 March 2012Additional files
Additional file 1:
Measured exchange fluxes in strains. Measured metabolite exchange fluxes for strains used in this study.
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Additional file 2:
Final glycerol concentration of WT and Δhac1 strains. Measured glycerol titers at end of fermentation for strains used in this study.
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Additional file 3:
Estimated exchange fluxes. Metabolite exchange fluxes as estimated by error-correction algorithm for strains in this study.
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Additional file 4:
Intracellular fluxes for metabolic network. Flux balance analysis estimates of internal fluxes for strains in thus study.
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Additional file 5:
Reporter TFs for WT protein secretion. Transcription factors activated by recombinant protein secretion in wild-type background.
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Additional file 6:
Reporter TFs for Δhac1 protein secretion. Transcription factors activated by recombinant protein secretion in Δhac1 background.
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Additional file 7:
Expression profiles for ribosomal proteins. mRNA concentrations for yeast ribosomal proteins as determined by DNA microarray.
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Additional file 8:
Oligonucleotides used in this study. PCR primers used for cloning and validation.
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Additional file 9:
Synthesized insulin precursor DNA sequence. DNA sequence for insulin precursor used in this study.
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