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Open Access Research article

Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress

Keith EJ Tyo12, Zihe Liu1, Dina Petranovic1 and Jens Nielsen1*

Author Affiliations

1 Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-41296 Göteborg, Sweden

2 Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd. Tech E136, Evanston, IL 60208, USA

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BMC Biology 2012, 10:16  doi:10.1186/1741-7007-10-16

Published: 1 March 2012

Additional files

Additional file 1:

Measured exchange fluxes in strains. Measured metabolite exchange fluxes for strains used in this study.

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Additional file 2:

Final glycerol concentration of WT and Δhac1 strains. Measured glycerol titers at end of fermentation for strains used in this study.

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Additional file 3:

Estimated exchange fluxes. Metabolite exchange fluxes as estimated by error-correction algorithm for strains in this study.

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Additional file 4:

Intracellular fluxes for metabolic network. Flux balance analysis estimates of internal fluxes for strains in thus study.

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Open Data

Additional file 5:

Reporter TFs for WT protein secretion. Transcription factors activated by recombinant protein secretion in wild-type background.

Format: DOC Size: 72KB Download file

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Open Data

Additional file 6:

Reporter TFs for Δhac1 protein secretion. Transcription factors activated by recombinant protein secretion in Δhac1 background.

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Additional file 7:

Expression profiles for ribosomal proteins. mRNA concentrations for yeast ribosomal proteins as determined by DNA microarray.

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Additional file 8:

Oligonucleotides used in this study. PCR primers used for cloning and validation.

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Additional file 9:

Synthesized insulin precursor DNA sequence. DNA sequence for insulin precursor used in this study.

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