Additional file 5.

Candidate gene primer and probe sets used in this study. Primers for the microsatellite markers were designed to amplify 150 to 350 base-pair genomic fragments containing length polymorphisms between surface fish and cavefish. Primers and fluorescent probes for Taqman and HybProbe genotyping methods were designed to detect single nucleotide polymorphisms between these two morphs.

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Yoshizawa et al. BMC Biology 2012 10:108   doi:10.1186/1741-7007-10-108