Additional file 7.
Alignment of the seven-transmembrane region of select opsin sequences. Columns of residues are shaded by similarity group conservation (defined by GeneDoc and the BLOSUM62 matrix) where black shows 100%, dark grey shows ≥80%, and light grey shows ≥60% similar residues in a column. Gene name abbreviations: MWS = medium-wavelength sensitive; RGR = retinal G protein-coupled receptor. Conserved sites of chromophore linkage at Lys296 (black circle), disulfide bridge formation at Cys110-Cys187 (black stars) and signal propagation at Glu134-Arg35-Tyr136 (black square) are indicated. Sites corresponding to the acidic counterion in the vertebrate pigments (Glu113) and the counterion in other species (Glu181) are also indicated (black triangles). Species are abbreviated as follows: Dm = Drosophila melanogaster; Es = Euprymna scolopes; Hs = Homo sapiens; Mlei = Mnemiopsis leidyi; Nv = Nematostella vectensis.
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Schnitzler et al. BMC Biology 2012 10:107 doi:10.1186/1741-7007-10-107