Quantitation of nuclear DNA loss and increase in a procyclic Trypanosoma brucei cell line following induction of expression of sister chromatid cohesion protein 1 (SCC1)-mutAB. Procyclic T. brucei before and 48 h after induction of expression of SCC1-mutAB were analyzed using 4', 6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) staining followed by color deconvolution and automated image analysis of DNA content and morphology. (A) Histograms of nuclear (left) and kinetoplast (right) DNA content of the uninduced cell line. Cells were categorized as normal (red), abnormally low (blue) or abnormally high (green) nuclear DNA content. (B) Histograms of nuclear (left) and kinetoplast (right) DNA content, at the same scale as (A), following induction for 48 h. Cells were categorized as normal, low or high nuclear DNA content based on the uninduced cell line. The proportion of cells with abnormally low or high nuclear DNA content has increased. Cells with abnormally high or low nuclear DNA content show no skew towards high or low kinetoplast DNA content.
Wheeler et al. BMC Biology 2012 10:1 doi:10.1186/1741-7007-10-1