Double labeling of kinetoplasts and nuclei with minor groove binding and base pair intercalating DNA stains. (A) Micrographs of procyclic Trypanosoma brucei labeled with 4', 6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI). (B) Promastigote Leishmania mexicana labeled with DAPI and SYBR green. (C) Choanomastigote Crithidia fasciculata labeled with Hoechst 33342 and PI. Lower panels show an enlarged view of individual cells. In each case the kinetoplast (K) appears brighter in the minor groove binding (MGB) stain image (DAPI or Hoechst 33342) than the base pair intercalating (BPI) stain image (PI or SYBR green). The reverse is true for the nucleus (N). For further examples of this effect see Additional file 1. Scale bar represents 10 μm.
Wheeler et al. BMC Biology 2012 10:1 doi:10.1186/1741-7007-10-1