Table 1

HPV L1 gene amplification by MY09/MY11 PCR primers followed by positive short or long nested PCR

HPV Type

MY09/MY11+

MY09/MY11-

Total isolates



Short

Long

Short_

Long





6

8

0

12

N

20

11

1

0

0

N

1

    16

40

0

28

N

68

    18

16

0

5

N

21

    31

11

0

4

N

15

32

3

0

0

N

3

    33

4

0

1

N

5

    35

6

1

0

N

7

    39

4

1

7

N

12

40

2

0

3

N

5

    45

4

0

5

N

9

    51

2

0

0

N

2

    52

9

0

16

N

25

53

2

1

0

N

3

54

5

0

13

N

18

    56

4

0

11

N

15

    58

6

0

3

N

9

    59

4

0

8

N

12

61

2

0

5

N

7

62

2

0

5

N

7

66

0

0

9

N

9

67

0

0

1

N

1

    68

0

0

1

N

1

69

0

0

2

N

2

70

1

0

5

N

6

71

0

0

2

N

2

72

4

0

2

N

6

73

4

0

7

N

11

74

0

0

1

N

1

81

3

0

4

N

7

83

2

2

1

N

5

84

2

0

1

N

3

86

0

0

4

N

4

87

0

0

1

N

1

91

0

0

1

N

1

Mixed

7

0

21

N

28


Total

158

5

189

352


MY09/MY11+ = 450 bp amplicon visualized on primary PCR gel.

MY09/MY11- = 450 bp amplicon not visualized on primary PCR gel.

Short = 150 bp or 190–200 bp nested PCR amplicon visualized.

Long = 380–395 bp nested PCR needed due to failure of short PCR.

N = not performed.

Underlined are "High-risk" HPVs targeted by Digene hc2 assay.

Lee et al. BMC Clinical Pathology 2009 9:3   doi:10.1186/1472-6890-9-3

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