A typical signature sequence excised from an electropherogram generated by direct automated DNA sequencing, including 23 bases of the GP5+ priming site (underlined). The template was generated by a pair of HiFi HPV nested PCR primers (equivalent to length flanked by GP6/MY11). A sequence of any 34 of these 53 bases confirms that this is an HPV-31 L1 gene DNA through BLAST sequence alignment algorithm. Any 50-base sequence in this region can be used for accurate genotyping of all known HPVs.
Lee et al. BMC Clinical Pathology 2009 9:3 doi:10.1186/1472-6890-9-3