Concordance of DNA diploidy on standard FC and ICS in a control but not in BAC.A: Normal gastric mucosa shows a diploid DNA histogram on standard FC as well as on ICS. Note that the diploid peak is sharp and narrow (CV = 4.18%) on standard FC and on ICS. There is no increased scatter of cells outside the peak. B: BAC shows a DNA diploid histogram on standard FC but a DNA aneuploid histogram on ICS. The top panel shows a DNA diploid (2N) peak on flow FC. The bottom panel on ICS shows no diploid peak but a wide and short aneuploid peak with peak DI of 1.76, as well as cells with different DI values but no cells with DNA content > 9N. (X-axis shows IOD values and the vertical lines mark DI values).
Huang et al. BMC Clinical Pathology 2008 8:5 doi:10.1186/1472-6890-8-5