Table 1

RNA recovery for each sample group

Procurement method

Total RNA (ng/μL)

Degradometer (range)

B-actin 3'/5' ratio

GAPDH 3'/5' ratio


Group 1

1,546.80

Orange – red 24.1% (19.52 – 28.69)

5.6

3.8

Group 2

49.27

Black 58.6% (56.32 – 60.37)

18.4

15

Group 3

59.96

Black 41.2% (40.92 – 41.55)

9.9

19.3


Measurements of recovery and metrics of degradation for each sample procurement method for RNA in each group. Group 1 = exfoliation/enrichment approach, Group 2 = FFPE + LCM method, Group 3 = Frozen tissue section + LCM. Classification of Degradometer parameters: Yellow = degradation between 8–16%, orange = degradation between 16–24%, red = degradation > 24% and black = extensive degradation from which non-reliable data may be generated.

Mojica et al. BMC Clinical Pathology 2007 7:7   doi:10.1186/1472-6890-7-7

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