Table 1

The effect of exposure to preservative solution for 20 hours on the viability of renal biopsy tissue assessed by MTT reduction and a histology score following CD 10 antibody staining. See text for abbreviations.

Preservative solution

Temp. of incubation

Duration of incubation

Mean optical density readings

% reduction in formazan formation

Histology score (PAS)

Immuno-staining score (CD 10)


1. NCS

1°C

20 hours

0.225

31.60%

12

2

2. PBS

1°C

20 hours

0.151

54.10%

12

2

3. MEM

1°C

20 hours

0.197

40.10%

7

2

4. MEM+NCS+Cells

1°C

20 hours

0.253

23.10%

10

2

5. Water

1°C

20 hours

0.01

97.00%

4

0

6. Soltran

1°C

20 hours

0.283

14.00%

8

3

7. MEM+NCS

1°C

20 hours

0.244

25.80%

9

3

8. None (Control)

37°C

0 minutes

0.329

-

11

3

9. NCS

37°C

20 hours

0.164

50.20%

5

1

10. PBS

37°C

20 hours

0.029

91.20%

4

1

11. MEM

37°C

20 hours

0.127

61.40%

6

1

12. MEM+NCS

37°C

20 hours

0.174

47.10%

4

1

13. MEM+NCS+Cells

37°C

20 hours

0.179

45.60%

4

1

14. Water

37°C

20 hours

0.007

97.90%

0

0

15. Soltran

37°C

20 hours

0.025

92.40%

3

1


Tagboto and Griffiths BMC Clinical Pathology 2007 7:5   doi:10.1186/1472-6890-7-5

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