Dihydrorhodamine (DHR) analysis of oxidation in peripheral blood neutrophils. In brief, after red blood cell lysis, neutrophils are loaded with DHR. After DHR loading, cells were stimulated for 15 mins with phorbol myristate acetate (PMA) and immediately analyzed by flow cytometry. DHR is a fluorescent dye taken up by neutrophils. When the neutrophils are stimulated and undergo respiratory burst the dye is oxidised with a shift of fluorescence to the right. Shown are (a) normal patient with unimodal shift of fluorescence after PMA stimulation; (b) the patient who has virtually absent shift in stimulated neutrophils consistent with X-linked CGD; (c) the mother of the patient demonstrating dual population of neutrophils in peripheral blood of X-linked CGD carriers.
Mclean-Tooke et al. BMC Clinical Pathology 2007 7:1 doi:10.1186/1472-6890-7-1