Comparison of Intact and Degraded FFPE Extracted RNAs. A. Electropherogram of RNA graded "Passed" by Asterand quality measures from cryopreserved tissue of a mirror image preparation of the FFPE sample being studied. One can see clear peaks for both 18S and 28S ribosomal RNA with low amount of noise between the peaks. C. Cryopreserved mirror sample graded "Failed" by Asterand quality measure. A lack of distinguishable peaks and also a large amount of fragments in the low molecular weight range indicates a large amount of degradation. B. & D. The right shows the reproducibility of the assay based on scattergrams of signal intensities from extracted FFPE RNA on replicate DASL assays and the obtained Pearson R2 correlation coefficient squared (R2). Both scattergrams demonstrate highly reproducible results.
Haller et al. BMC Clinical Pathology 2006 6:9 doi:10.1186/1472-6890-6-9