Comparison of Intact and Degraded Cryopreserved RNAs. A. Electropherogram constructed from Bioanalyzer microchip electrophoresis of RNA extracted from cryopreserved tissue graded "Passed" by Asterand quality assessment method. Prominent bands can be seen as sharp peaks at 18S and 28S indicating highly intact RNA. C. Sample that did not pass Asterand quality measures. Electropherogram shows an 18S peak larger than the 28S peak and an increased amount of noise throughout the electropherogram. B. & D. The panels on the right show scattergrams of the signal intensities of individual genes for replicate analyses of each sample, and the associated Pearson correlation coefficient squared (R2). Both samples provided reproducible results.
Haller et al. BMC Clinical Pathology 2006 6:9 doi:10.1186/1472-6890-6-9