Figure 4.

Immunohistochemical staining of liver neutrophils. Mice were pretreated with vehicle (i.e. normal saline), DMPP (1 mg/kg), or nicotine (1 mg/kg) prior to the onset of ischemia (90 min), followed by 24 h of reperfusion. Immunostaining of the liver sections was performed using a specific anti-neutrophil antibody. A matched antibody isotype IgG was used as a negative control antibody. Neutrophils are presented by a dark brown stain. Images are representative of three separate experiments with similar results.

Crockett et al. BMC Clinical Pathology 2006 6:3   doi:10.1186/1472-6890-6-3
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