Table 1

Comparison of HER-2/neu status assessed by FISH and Real-Time PCR in 39 breast cancer samples deemed 2+ by IHC

Sample number

FISH

Real-Time PCR


CEP 17

HER-2

Ratio

Status

Ratio

Status


1

1.83

1.83

1.00

-

1.33

-

2

1.45

1.60

1.10

-

0.48

-

3

1.18

1.23

1.04

-

0.65

-

4

1.35

1.27

0.94

-

1.3

-

5

1.48

1.75

1.18

-

1.5

-

6

1.37

1.37

1.00

-

1.74

-

7

2.40

3.21

1.34

-

0.66

-

8

1.88

1.73

0.92

-

1.35

-

9

6.22

5.85

0.94

-

1.46

-

10

1.77

1.75

0.99

-

1.62

-

11

1.28

1.28

1.00

-

1.49

-

12

1.72

1.68

0.98

-

1.52

-

13

1.53

1.50

0.98

-

1.42

-

14

1.80

1.78

0.99

-

1.43

-

15

3.30

3.22

0.98

-

1.18

-

16

2.47

2.45

0.99

-

1.59

-

17

1.73

1.82

1.05

-

0.41

-

18

1.70

1.73

1.02

-

1.53

-

19

1.83

1.93

1.05

-

0.35

-

20

3.03

3.55

1.17

-

1.58

-

21

1.97

1.55

0.79

-

1.65

-

22

1.68

1.72

1.02

-

1.60

-

23

1.40

1.20

0.86

-

1.39

-

24

1.27

1.80

1.42

-

1.77

-

25

1.20

1.18

0.98

-

1.68

-

26

1.07

1.07

1.00

-

0.44

-

27

1.10

1.08

0.98

-

0.76

-

28

1.98

2.40

1.21

-

1.49

-

29

1.85

2.58

1.39

-

1.96

-

30

2.70

3.22

1.19

-

1.76

-

31

1.95

1.97

1.01

-

1.84

-

32

4.17

6.85

1.64

-

1.67

-


33

1.92

2.00

1.04

-

2.07

+

34

1.77

7.13

4.03

+

1.83

-

35

1.93

4.92

2.55

+

1.51

-


36

1.92

6.77

3.53

+

3.91

+

37

1.77

16.70

9.44

+

11.10

+

38

2.00

5.87

2.94

+

2.09

+

39

2.00

8.15

4.08

+

2.39

+


CEP 17 = mean/cell of the chromosome 17 centromere probe signals HER-2 = mean/cell of the HER-2/neu probe signals

FISH Ratio = the ratio between HER-2 and CEP 17

Real-Time PCR Ratio = the normalized ratio between HER-2 and the reference gene (gastrin)

"+" = positive, "-"=negative. Cases (8%) that were discordant between PCR and FISH assays are highlighted.

Nistor et al. BMC Clinical Pathology 2006 6:2   doi:10.1186/1472-6890-6-2

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